胱天蛋白酶3(CASP3)檢測試劑盒(酶聯免疫吸附試驗法)
ELISA Kit for Caspase 3 (CASP3)
CPP32; CPP32B; SCA1; Apoptain; Yama; Apoptosis-Related Cysteine Peptidase; Cysteinyl Aspartate Specific Proteinases 3; SREBP cleavage activity 1
- 編號USEA626Bo
- 物種Bos taurus; Bovine (Cattle，牛) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍0.156-10ng/mL
- 靈敏度最小可檢測劑量小于等于0.055ng/mL.
- 樣本類型Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1588 ¥ 2268 ¥ 10206 ¥ 19278 ¥ 158760
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產品包裝(模擬)
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產品包裝(模擬)
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實驗結果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測胱天蛋白酶3(CASP3)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
精密度
精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
穩(wěn)定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數。
實驗原理
將胱天蛋白酶3(CASP3)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的胱天蛋白酶3(CASP3)與連接于固相載體上的抗體結合，然后加入生物素化的胱天蛋白酶3(CASP3)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的胱天蛋白酶3(CASP3)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務
相關產品
| 編號 | 適用物種：Bos taurus; Bovine (Cattle，牛) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPA626Bo01 | 胱天蛋白酶3(CASP3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA626Bo01 | 胱天蛋白酶3(CASP3)多克隆抗體 | WB |
| UMAA626Bo21 | 胱天蛋白酶3(CASP3)單克隆抗體 | WB; IHC; ICC; IP. |
| USEA626Bo | 胱天蛋白酶3(CASP3)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA626Bo | 胱天蛋白酶3(CASP3)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
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| Journal of Ethnopharmacology | Ethnopharmacological impact of Melaleuca rugulosa (Link) Craven leaves extract on liver inflammation [Pubmed:35337921] |
| Eur J Pharmacol | Harmine prevents 3-nitropropionic acid-induced neurotoxicity in rats via enhancing NRF2-mediated signaling: Involvement of p21 and AMPK [Pubmed:35623405] |
| Gels | QbD Supported Optimization of the Alginate-Chitosan Nanoparticles of Simvastatin in Enhancing the Anti-Proliferative Activity against Tongue Carcinoma [Pubmed:35200484] |
| Naunyn-Schmiedeberg's Archives | Inflammatory Modulation of miR-155 Inhibits Doxorubicin?Induced Testicular Dysfunction Via Sirt1/Foxo1 Pathway: Insight to the Role of Acacetin and Bacillus?… [Pubmed:35715546] |
| Antioxidants | Therapeutic Effects of Bee Bread on Obesity-Induced Testicular-Derived Oxidative Stress, Inflammation, and Apoptosis in High-Fat Diet Obese Rat Model?… [Pubmed:35204140] |
| Value Health Sci | Oksidatif, Apoptotik ve ?nflamatuar Sinyal Yolaklar? üzerinden C6 Glioblastoma Hücrelerindeki ML351'in Antiproliferatif Etkileri [] |
| Impact de l'incorporation d'acides gras à chaine moyenne, de suppléments de levures et d'avoine nue sur la santé intestinale ainsi que sur la consommation?… [] | |
| Biointerface Research in Applied Chemistry | Promising Therapeutic Efficacy of Trigonella-foenum graecum and Bone Marrow-Derived Mesenchymal Stem Cells on Skeletal Muscle Atrophy in Experimental?… [] |
| Arab Journal of Nuclear Sciences and Applications | Potential activity of silver/hydroxyapatite nano composite against damaged liver consequence to irradiation [] |
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