蛋白酶K(PROK)活性蛋白
Active Proteinase K (PROK)
Endopeptidase K; Tritirachium alkaline proteinase; Protease K; Tritirachium alkaline proteinase; Tritirachium album serine proteinase
- 編號(hào)UAPX263Ge51
- 物種Pan-species (General，通用) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 5% Trehalose.
- 性狀凍干粉
- 純度> 95%
- 等電點(diǎn)7.1
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Proteinase K is a typical member of the subtilisin family of proteinases .It exhibits broad substrate specificity and degrades many proteins in the native state, even in the presence of detergents.The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. Thus,the activity of recombinant Proteinase K measued by hydrolyzing casein.The reaction was performed in adding 200μl recombinant Proteinase K(dilued by 0.01mol/L Tris-HCL,pH 8.0) to 200μl 10g/L casein ,rapidly mixing,incubated for 5min at 55 ℃,stop the reaction with 400μl 0.4mol/L TCA.Clarify by filtration through a 0.45 m filter or by centrifugation. Read the supernatant an 275nm. The blank use 200μl 0.01mol/L Tris-HCL,pH 8.0 replace the Proteinase K. One unit is described as that amount of enzyme that liberates 1 μg of L- tyrosine within one minute at 55°C using casein as a substrate.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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| 編號(hào) | 適用物種：Pan-species (General，通用) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UEPX263Ge51 | 蛋白酶K(PROK)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPX263Ge51 | 蛋白酶K(PROK)活性蛋白 | Cell?culture;?Activity?Assays. |
