谷丙轉(zhuǎn)氨酶(ALT)單克隆抗體
Monoclonal Antibody to Alanine Aminotransferase (ALT)
GPT; ALAT; SGPT; AAT1; GPT1; Serum Glutamic Pyruvic Transaminase; Alanine Transaminase; Glutamate pyruvate transaminase 1; Glutamic--alanine transaminase 1
- 編號UMAA207Hu21
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)C4
- Ig亞型 IgG1 Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPA207Hu03-谷丙轉(zhuǎn)氨酶(ALT)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/ml
- 且適物種Rattus norvegicus (Rat，大鼠)
- 應(yīng)用WB; IHC
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 324 ¥ 756 ¥ 1080 ¥ 2700 ¥ 10800
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Sample: Human Kidney Tissue; Primary Ab: 20μg/ml Mouse Anti-Human ALT Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Human Liver Tissue;
Primary Ab: 20μg/ml Mouse Anti-Human ALT Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Skeletal muscle Tissue;
Primary Ab: 20μg/ml Mouse Anti-Human ALT Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Liver Tissue
Primary Ab: 20μg/ml Mouse Anti-Human ALT Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 20μg/ml Mouse Anti-Human ALT Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Skeletal muscle Tissue
Primary Ab: 20μg/ml Mouse Anti-Human ALT Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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Western Blot; Sample: Lane1: Rat Liver lysate; Lane2: Rat Skeletal muscle lysate Primary Ab: 2μg/ml Mouse Anti-Human ALT Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對ALT的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別ALT。
用法
Western blottin:g: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
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| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA207Hu02 | 谷丙轉(zhuǎn)氨酶(ALT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA207Hu03 | 谷丙轉(zhuǎn)氨酶(ALT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA207Hu02 | 谷丙轉(zhuǎn)氨酶(ALT)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA207Hu01 | 谷丙轉(zhuǎn)氨酶(ALT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA207Hu01 | 谷丙轉(zhuǎn)氨酶(ALT)多克隆抗體 | WB; IHC |
| UPAA207Hu03 | 谷丙轉(zhuǎn)氨酶(ALT)多克隆抗體 | WB; IHC |
| UPAA207Hu02 | 谷丙轉(zhuǎn)氨酶(ALT)多克隆抗體 | WB; IHC |
| ULAA207Hu71 | 谷丙轉(zhuǎn)氨酶(ALT)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| ULAA207Hu81 | 谷丙轉(zhuǎn)氨酶(ALT)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| UMAA207Hu21 | 谷丙轉(zhuǎn)氨酶(ALT)單克隆抗體 | WB; IHC |
| UMAA207Hu24 | 谷丙轉(zhuǎn)氨酶(ALT)單克隆抗體 | WB |
| UMAA207Hu23 | 谷丙轉(zhuǎn)氨酶(ALT)單克隆抗體 | IHC |
| UMAA207Hu25 | 谷丙轉(zhuǎn)氨酶(ALT)單克隆抗體 | IHC |
| UMAA207Hu22 | 谷丙轉(zhuǎn)氨酶(ALT)單克隆抗體 | WB; IHC |
| UMAA207Hu27 | 谷丙轉(zhuǎn)氨酶(ALT)單克隆抗體 | IHC |
| UMAA207Hu29 | 谷丙轉(zhuǎn)氨酶(ALT)單克隆抗體 | IHC |
| ULAA207Hu72 | 谷丙轉(zhuǎn)氨酶(ALT)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UMEA207Hu | 谷丙轉(zhuǎn)氨酶(ALT)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USEA207Hu | 谷丙轉(zhuǎn)氨酶(ALT)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA207Hu | 谷丙轉(zhuǎn)氨酶(ALT)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
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| Gastroenterol Res Pract | Assessing the Effect of Leptin on Liver Damage in Case of Hepatic Injury Associated with Paracetamol Poisoning [PubMed: 26697061] |
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| Rolle von CMKLR1 und dessen Liganden bei der nicht-alkoholischen Fettlebererkrankung [Regensburg:Source] | |
| Cumhuriyet Medical Journal | The valuable effects of potent antioxidant curcumin in cisplatin induced liver and kidney injury [DOI:Source] |
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| Scientific Reports | Brg1 promotes liver regeneration after partial hepatectomy via regulation of cell cycle [Pubmed: 30787318] |
| Brain Behavior and Immunity | Corticosterone-mediated microglia activation affects dendritic spine plasticity and motor learning functions in minimal hepatic encephalopathy [Pubmed: 31437533] |
| Biomed Research International | Liraglutide Attenuates Nonalcoholic Fatty Liver Disease by Modulating Gut Microbiota in Rats Administered a High-Fat Diet [Pubmed: 32149099] |
| Frontiers in Immunology | CD47 Deficiency in Mice Exacerbates Chronic Fatty Diet-Induced Steatohepatitis Through Its Role in Regulating Hepatic Inflammation and Lipid Metabolism [Pubmed: 32158445] |
| Nanomaterials | Biological Safety and Biodistribution of Chitosan Nanoparticles [Pubmed: 32340313] |
| European Journal of Biology | Effects of Tempol in Lipopolysaccharide-Induced Liver Injury [] |
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| International Immunopharmacology | Dimethyl fumarate ameliorates autoimmune hepatitis in mice by blocking NLRP3 inflammasome activation [Pubmed:35605433] |
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